Hereditary spherocytosis

Clinical assessment, including personal and family history of anaemia, jaundice, gall stones.

FBC, blood film, reticulocyte count; bilirubin, LD.

Haptoglobin may assist to confirm haemolysis.

Direct antiglobulin test to exclude autoimmune haemolysis.

Auto haemolysis test or Osmotic fragility may be useful if diagnosis uncertain.

Flow cytometry evaluation is diagnostic.

Family studies, as appropriate.