Burkholderia pseudomallei antibodies - serum

Last updated: Sunday, 21, May, 2006

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Item Process
Specimen

5-10 mL blood in plain tube.

Method

IHA, IFA, EIA

Application

The diagnosis of suspected melioidosis in patients who have lived in, or visited, endemic areas (primarily SE Asia and northern Australia) and have presented with pneumonia, secondary abscesses or unexplained acute septicaemic episodes.

Culture of Burkholderia pseudomallei from blood, sputum, or tissue is more rapid and reliable means of diagnosing infection.

Interpretation

The IHA test is of limited clinical value. In a non-endemic area, a single titre of 40 or greater is regarded as positive, but a four-fold rise in titre is required for a definitive diagnosis.

A negative result does not exclude the disease. 

EIA and IFA tests are much more sensitive.

Reference

Gilligan PH et al. In: Murray PR et al eds.  Manual of Clinical Microbiology. 8th ed. ASM Press 2003