Bordetella pertussis detection - nasopharyngeal secretions
Last updated: Friday, 19, March, 2004
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Nasopharyngeal aspirate or swab; aspirate is preferred.
PCR testing may be performed on a throat swab (check with local laboratory).
Culture, nucleic acid probe after PCR amplification.
A positive result confirms a clinical diagnosis of whooping cough.
Nucleic acid detection after PCR amplification is more sensitive than culture.
Testing is usually negative if symptoms have been present for more than one month.
Farrell DJ et al. J Clin Microbiol 1999; 37: 606-610.