Direct antiglobulin test (DAT)

Last updated: Thursday, 01, April, 2004

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Item Process
Specimen5 mL blood in EDTA tube.
MethodPolyspecific anti-human globulin serum added to patient’s washed red cells. The test is positive if agglutination is observed.
Application

Investigation of possible immune haemolysis.

Detection of antibody and/or complement on red cell surface.

ReferenceFord D. Aust J Med Lab Sci 1995; 16: 67-70.