Donath Landsteiner antibody
Last updated: Wednesday, 05, December, 2007
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|Specimen||Collection by laboratory staff into 2 plain tubes, one at 37°C and one at 4°C.|
|Method||Supernatant serum examined for evidence of red cell lysis, comparing a tube incubated at 4°C then 37°C with a tube maintained at 37°C.|
| Reference Interval||No lysis in either tube.|
|Application||Diagnosis of paroxysmal cold haemoglobinuria.|
The Donath Landsteiner antibody is an IgG autoantibody that binds to red cells in the cold and fixes complement; lysis occurs when cells are warmed to 37°C.
Presence of the antibody is diagnostic for paroxysmal cold haemoglobinuria. This rare disorder may occur in syphilis and after viral infections in children.
|Reference||Mitchell Lewis S, Bain BJ, Bates I. Dacie and Lewis Practical Haematology. Churchill Livingstone. 10th ed 2006.|