Haemoglobin S (HbS)

Last updated: Monday, 22, March, 2004

MBS Online Search Page: Click here for details

Item Process

5 mL blood in any anticoagulant.

  1. Examination of blood film incubated with reducing agent.
  2. Qualitative solubility test in presence of reducing agent.
  3. Haemoglobin electrophoresis  and HPLC.

Investigation where sickle cell disease (SS) or trait (AS) is suspected.

HbS may be present with other abnormalities (eg, HbS/thalassaemia).

Women from ethnic backgrounds where sickle cell disease is common should be screened prior to, or early in pregnancy. This is to identify couples who are at risk of having a child with sickle cell disease so that prenatal testing and counselling can be offered.
At risk ethnic groups include individuals derived from Mediterranean, Arabic, Middle Eastern and West African regions.

Specific HbS testing is required since individuals with sickle cell trait may not have any abnormalities on the full blood count, which cannot be relied on as a screening test.


Sickle cell tests depend on decreased solubility of HbS at low oxygen tensions or on characteristic mobility of HbS on electrophoresis.


Working Party of the General Haematology Task Force of the British Committee for Standards in Haematology. Br J Haematol 1998; 101: 783-792.