Indirect antiglobulin test

Last updated: Friday, 02, April, 2004
Item Process
Specimen

5 mL blood in plain tube.

Method

Patient’s serum incubated with test red cells; red cells washed, incubated with polyvalent anti-human globulin serum and observed for agglutination.

Application

Detection of red cell (blood group) antibodies in serum. Screen for red cell antibodies in pregnancy and prior to compatibility testing (cross match).

Detection and definition of antibodies in immune haemolytic anaemia.

See also Blood group antibody screen and compatibility testing.

Interpretation

Agglutination indicates presence of antibody (or complement) bound to red cell surface.

Reference

Mollison PL. Blood Transfusion in Clinical Medicine. 9th ed. Blackwell Scientific 1993.