Lymphocyte proliferative response

Last updated: Monday, 22, March, 2004

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Item Process
Specimen

30 mL blood in preservative-free heparin.

Method

The proliferative response to specific antigen or mitogen (eg, phytohaemagglutinin [PHA], concanavalin A, pokeweed mitogen) is demonstrated by uptake of tritiated thymidine or expression of differentiation markers.

Reference Interval

Consult pathologist.

Application

Assessment of cell mediated immune competence.

May be of use in assessment of histocompatibility differences in organ transplantation.

Rarely indicated as a diagnostic test outside tranplantation area.

Interpretation

Impaired T lymphocyte proliferative response to mitogen or to HLA-incompatible stimulator cells indicates depressed cell mediated immunity.

Reference

Fletcher M et al. In: Rosen NR et al eds. Manual of Clinical Laboratory Immunology. 4th ed. ASM Press 1997.