C-Peptide suppression test
Last updated: Thursday, 08, April, 2004
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5 mL blood in EDTA tube plus 5 mL blood in lithium heparin or fluoride/oxalate tube.
After an overnight fast, the patient is given an infusion of insulin (0.125 U/kg) over 60 minutes.
Specimens are collected immediately prior to the infusion, then every 30 minutes for two hours.
Glucose and C-peptide analyses are performed.
The test must be performed with continuous medical monitoring, and may need to be terminated if significant clinical hypoglycaemia occurs.
| Reference Interval|
If glucose levels fall to <2.2 mmol/L, trough C-peptide should be <0.4 nmol/L (1.2 µg/L).
Diagnosis of insulinoma, though now largely replaced by other protocols.
If C-peptide does not fall to within the reference interval in a patient who becomes hypoglycaemic the patient has an autonomous source of endogenous insulin.
A decrease in the percentage fall from the baseline value may also be used as a diagnostic criterion as the occasional patient with an insulinoma may have a low baseline level.
Service FJ et al. J Clin Endocrinol Metab 1992; 74: 204-210.
Grant CS. Baillieres Clin Gastroenterol 1996; 10(4): 645-671.