Complement CH50 or CH100 - serum

Last updated: Monday, 29, May, 2006

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Item Process
Specimen

10 mL blood in plain tube.

The specimen should be placed on melting ice and transported immediately to the laboratory.

Method

Lysis of haemolysin-treated sheep red cells in solution or agarose.

CH50 is a tube method, measuring 50% cell lysis. CH100 is a screening plate method measuring 100% cell lysis.

Reference Interval

Consult pathologist.

Application

A screening test for patients with suspected inherited complement component deficiency. The tests are of no value in monitoring complement activation in acquired disorders eg, SLE.

Interpretation

Incorrect specimen collection is the most common cause of reduced levels. Reduced levels may also indicate deficiency in one of the components of complement. Levels will be variably reduced in acquired disorders associated with complement activation.

Reference

Ruddy S. In: Rose NR et al eds. Manual of Clinical Laboratory Immunology. 4th ed. ASM Press 1997.

Wen L et al. Journal of Allergy and Clinical Immunology. 2004; 113: 585-93.