Hydroxyproline - urine

Last updated: Monday, 22, March, 2004

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Item Process

2 hour timed urine collection after an overnight fast.

The patient should not consume meat or gelatine-containing products (eg, ice cream) for the 24 hours prior to the collection.


Spectrophotometry. Results are expressed as a ratio to creatinine.

Reference Interval

Dependent on age and lean body mass. Adult: <35 mmol/mol creatinine.


Assessment of bone resorption eg, in the monitoring of Paget’s disease and osteoporosis.

Has largely been replaced by other markers of bone turnover.


Hydroxyproline output indicates the level of active bone turnover and associated collagen catabolism.


Bettica P et al. Clin Chem 1992; 38: 2313-2318.