FISH (Fluorescence in situ hybridisation)
Last updated: Monday, 06, August, 2007
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Blood, bone marrow (collected in sterile, preservative-free heparin), tumour, placenta, foetal tissue or amniotic fluid.
Specimens must be delivered fresh to the laboratory.
Slides are prepared directly from the specimen or after cell culture.
Metaphase or interphase preparations are used.
Specific fluorescent DNA probes are hybridised to chromosomal DNA. Multiple loci can be probed simultaneously.
FISH is a sensitive and useful adjunct to cytogenetic testing for the detection of abnormalities of chromosomal structure or numbers (eg, deletions, translocations, duplications, aneuploidy). It is often the method of choice for detection of microdeletions.
In disorders such as CLL or multiple myeloma where it may be difficult to induce malignant cells to divide, interphase FISH can detect abnormalities of prognostic significance.
FISH provides a sensitive method to identify chromosomal abnormalities and rapid assessment of large numbers of cells (>500). It is highly accurate, for the probe, in identifying the origin of the chromosome or chromosomal fragment under investigation.
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