Last updated: Wednesday, 28, April, 2004
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5 mL blood or 2 mL bone marrow aspirate in lithium heparin, ACD or EDTA.
Fresh, unfixed lymphoid or other tissue in sterile tissue culture medium.
|Method||Cell suspension labelled with fluorescent-conjugated antibodies and analysed on a flow cytometer.|
| Reference Interval||Consult pathologist.|
Assessment of lymphoid cell subsets especially CD4 numbers in patients with HIV infection;
May also be used to count reticulocytes;
Positive staining indicates expression of the relevant antigen.
Report will identify and quantitate cell types present with an interpretative comment from the pathologist.
Bauer KD et al eds. Clinical Flow Cytometry - Principles and Application. Williams and Wilkins 1993.
Stewart CC et al eds. Immunophenotyping. Wiley-Liss 2000.
Roger SR. Hematol Oncol Clin North Am 2002; 16.